A Conserved Circular Network of Coregulated Lipids Modulates Innate Immune Responses.

ABSTRACT: Lipid composition affects the biophysical properties of membranes that provide a platform for receptor-mediated cellular signaling. To study the regulatory role of membrane lipid composition, we combined genetic perturbations of sphingolipid metabolism with the quantification of diverse steps in Toll-like receptor (TLR) signaling and mass spectrometry-based lipidomics. Membrane lipid composition was broadly affected by these perturbations, revealing a circular network of coregulated sphingolipids and glycerophospholipids. This evolutionarily conserved network architecture simultaneously reflected membrane lipid metabolism, subcellular localization, and adaptation mechanisms. Integration of the diverse TLR-induced inflammatory phenotypes with changes in lipid abundance assigned distinct fun

SUBMITTER: Koberlin MS 

PROVIDER: S-EPMC4523684 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature