Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress.

ABSTRACT: The TET enzymes convert methylcytosine to the newly discovered base hydroxymethylcytosine. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain, and results lack in vivo models. Here we show a global decrease of hydroxymethylcytosine in cells treated with buthionine sulfoximine, and in mice depleted for the major antioxidant enzymes GPx1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in coding genes, and intriguingly in microRNA genes, both involved in response to oxidative stress. These results thus suggest a profound effect of in vivo oxidative stress on the global hydroxymethylome.

SUBMITTER: Delatte B 

PROVIDER: S-EPMC4523844 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature