Cell- and Protein-Directed Glycosylation of Native Cleaved HIV-1 Envelope.

ABSTRACT:

Unlabelled

The gp120/gp41 HIV-1 envelope glycoprotein (Env) is highly glycosylated, with up to 50% of its mass consisting of N-linked glycans. This dense carbohydrate coat has emerged as a promising vaccine target, with its glycans acting as epitopes for a number of potent and broadly neutralizing antibodies (bnAbs). Characterizing the glycan structures present on native HIV-1 Env is thus a critical goal for the design of Env immunogens. In this study, we used a complementary, multistep approach involving ion mobility mass spectrometry and high-performance liquid chromatography to comprehensively characterize the glycan structures present on HIV-1 gp120 produced in peripheral blood mononuclear cells (PBMCs). The capacity of different expression systems, including pseudoviral partic

SUBMITTER: Pritchard LK 

PROVIDER: S-EPMC4524065 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature