Enhancing the Detection of Giardia duodenalis Cysts in Foods by Inertial Microfluidic Separation.

ABSTRACT: The sensitivity and specificity of current Giardia cyst detection methods for foods are largely determined by the effectiveness of the elution, separation, and concentration methods used. The aim of these methods is to produce a final suspension with an adequate concentration of Giardia cysts for detection and a low concentration of interfering food debris. In the present study, a microfluidic device, which makes use of inertial separation, was designed and fabricated for the separation of Giardia cysts. A cyclical pumping platform and protocol was developed to concentrate 10-ml suspensions down to less than 1 ml. Tests involving Giardia duodenalis cysts and 1.90-μm microbeads in pure suspensions demonstrated the specificity of the microfluidic chip for cysts over smaller nonspecific parti

SUBMITTER: Ganz KR 

PROVIDER: S-EPMC4524145 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature