Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas9.

ABSTRACT: The human genome contains millions of DNA regulatory elements and a large number of gene clusters, most of which have not been tested experimentally. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9) programed with a synthetic single-guide RNA (sgRNA) emerges as a method for genome editing in virtually any organisms. Here we report that targeted DNA fragment inversions and duplications could easily be achieved in human and mouse genomes by CRISPR with two sgRNAs. Specifically, we found that, in cultured human cells and mice, efficient precise inversions of DNA fragments ranging in size from a few tens of bp to hundreds of kb could be generated. In addition, DNA fragment duplications and deletions could also be generated by CRISPR thr

SUBMITTER: Li J 

PROVIDER: S-EPMC4524425 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature