Transcriptome Profiling of Developing Murine Lens Through RNA Sequencing.

ABSTRACT:

Purpose

Transcriptome is the entire repertoire of transcripts present in a cell at any particular time. We undertook a next-generation whole transcriptome sequencing approach to gain insight into the transcriptional landscape of the developing mouse lens.

Methods

We ascertained mouse lenses at six developmental time points including two embryonic (E15 and E18) and four postnatal stages (P0, P3, P6, and P9). The ocular tissue at each time point was maintained as two distinct pools serving as biological replicates for each developmental stage. The mRNA and small RNA libraries were paired-end sequenced on Illumina HiSeq 2000 and subsequently analyzed using bioinformatics tools.

Results

Mapping of mRNA and small RNA libraries generated 187.56 and 154.22 million paired-end

SUBMITTER: Khan SY 

PROVIDER: S-EPMC4525677 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature