Myocyte Dedifferentiation Drives Extraocular Muscle Regeneration in Adult Zebrafish.

ABSTRACT:

Purpose

The purpose of this study was to characterize the injury response of extraocular muscles (EOMs) in adult zebrafish.

Methods

Adult zebrafish underwent lateral rectus (LR) muscle myectomy surgery to remove 50% of the muscle, followed by molecular and cellular characterization of the tissue response to the injury.

Results

Following myectomy, the LR muscle regenerated an anatomically correct and functional muscle within 7 to 10 days post injury (DPI). Following injury, the residual muscle stump was replaced by a mesenchymal cell population that lost cell polarity and expressed mesenchymal markers. Next, a robust proliferative burst repopulated the area of the regenerating muscle. Regenerating cells expressed myod, identifying them as myoblasts. However, both immun

SUBMITTER: Saera-Vila A 

PROVIDER: S-EPMC4525682 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature