Poly peak parser: Method and software for identification of unknown indels using sanger sequencing of polymerase chain reaction products.

ABSTRACT:

Background

Genome editing techniques, including ZFN, TALEN, and CRISPR, have created a need to rapidly screen many F1 individuals to identify carriers of indels and determine the sequences of the mutations. Current techniques require multiple clones of the targeted region to be sequenced for each individual, which is inefficient when many individuals must be analyzed. Direct Sanger sequencing of a polymerase chain reaction (PCR) amplified region surrounding the target site is efficient, but Sanger sequencing genomes heterozygous for an indel results in a string of "double peaks" due to the mismatched region.

Results

To facilitate indel identification, we developed an online tool called Poly Peak Parser (available at http://yost.genetics.utah.edu/software.php) that is able to

SUBMITTER: Hill JT 

PROVIDER: S-EPMC4525701 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature