Protein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellum.

ABSTRACT: The eukaryotic flagellum is a large structure into which specific constituent proteins must be targeted, transported and assembled after their synthesis in the cytoplasm. Using Trypanosoma brucei and a proteomic approach, we have identified and characterized a novel set of adenylate kinase proteins that are localized to the flagellum. These proteins represent unique isoforms that are targeted to the flagellum by an N-terminal extension to the protein and are incorporated into an extraaxonemal structure (the paraflagellar rod). We show that the N-terminal extension is both necessary for isoform location in the flagellum and sufficient for targeting of a green fluorescent protein reporter protein to the flagellum. Moreover, these N-terminal extension sequences are conserved in evolution and

SUBMITTER: Pullen TJ 

PROVIDER: S-EPMC452581 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature