Spatial and temporal regulation of Condensins I and II in mitotic chromosome assembly in human cells.

ABSTRACT: Two different condensin complexes make distinct contributions to metaphase chromosome architecture in vertebrate cells. We show here that the spatial and temporal distributions of condensins I and II are differentially regulated during the cell cycle in HeLa cells. Condensin II is predominantly nuclear during interphase and contributes to early stages of chromosome assembly in prophase. In contrast, condensin I is sequestered in the cytoplasm from interphase through prophase and gains access to chromosomes only after the nuclear envelope breaks down in prometaphase. The two complexes alternate along the axis of metaphase chromatids, but they are arranged into a unique geometry at the centromere/kinetochore region, with condensin II enriched near the inner kinetochore plate. This region-spe

SUBMITTER: Ono T 

PROVIDER: S-EPMC452584 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature