DNA-Microarray-based Genotyping of Clostridium difficile.

ABSTRACT:

Background

Clostridium difficile can cause antibiotic-associated diarrhea and a possibility of outbreaks in hospital settings warrants molecular typing. A microarray was designed that included toxin genes (tcdA/B, cdtA/B), genes related to antimicrobial resistance, the slpA gene and additional variable genes.

Results

DNA of six reference strains and 234 clinical isolates from South-Western and Eastern Germany was subjected to linear amplification and labeling with dUTP-linked biotin. Amplicons were hybridized to microarrays providing information on the presence of target genes and on their alleles. Tested isolates were assigned to 37 distinct profiles that clustered mainly according to MLST-defined clades. Three additional profiles were predicted from published genome sequen

SUBMITTER: Gawlik D 

PROVIDER: S-EPMC4526300 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature