Definition of a pool of epitopes that recapitulates the T cell reactivity against major house dust mite allergens.

ABSTRACT:

Background

Allergens from house dust mites (HDM) are a common cause of asthma. Der p and Der f from Dermatophagoides sp. are strong immunogens in humans. Allergen extracts are used to study T helper (Th2) cell responses to HDM, which are implicated in the development and regulation of allergic disease.

Objective

To define an epitope mixture that recapitulates, and might substitute for, HDM extract in terms of detecting and characterizing Th2 cell responses.

Methods

Peripheral blood mononuclear cells (PBMC) from 52 HDM allergic and 10 non-allergic individuals were stimulated with HDM extracts and assayed with a set of 178 peptides spanning mite allergens group Der p 1, 2, 23 and Der f group 1 and 2 allergens. A pool of the most dominant T cell epitopes identified in th

SUBMITTER: Hinz D 

PROVIDER: S-EPMC4526447 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature