Interaction between innate immunity and Ro52-induced antibody causes Sjogren's syndrome-like disorder in mice.

ABSTRACT:

Objectives

Autoantibodies reactive with Ro52 are often found in sera of patients with Sjögren's syndrome (SS). This study was undertaken to investigate the role of Ro52-induced immune responses in pathogenesis of SS.

Methods

New Zealand Mixed (NZM) 2758 mice were immunised with Ro52 in alum adjuvant. Control mice were immunised either with maltose-binding protein or injected with alum alone. Mice were monitored for anti-Ro52 antibody, sialoadenitis and pilocarpine-induced salivation. Antibody binding to salivary gland (SG) cells was analysed in vivo and in vitro by immunofluorescence. Sera from immunised mice were passively transferred into untreated or alum injected NZM2758 mice.

Results

By day 30 post-immunisation, Ro52 immunised mice generated immunoprecipitating a

SUBMITTER: Szczerba BM 

PROVIDER: S-EPMC4526450 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature