External Quality Assessment for the Detection of Measles Virus by Reverse Transcription-PCR Using Armored RNA.

ABSTRACT: In recent years, nucleic acid tests for detection of measles virus RNA have been widely applied in laboratories belonging to the measles surveillance system of China. An external quality assessment program was established by the National Center for Clinical Laboratories to evaluate the performance of nucleic acid tests for measles virus. The external quality assessment panel, which consisted of 10 specimens, was prepared using armored RNAs, complex of noninfectious MS2 bacteriophage coat proteins encapsulated RNA of measles virus, as measles virus surrogate controls. Conserved sequences amplified from a circulating measles virus strain or from a vaccine strain were encapsulated into these armored RNAs. Forty-one participating laboratories from 15 provinces, municipalities, or autonomous re

SUBMITTER: Zhang D 

PROVIDER: S-EPMC4526687 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature