Proteogenomic analysis of psoriasis reveals discordant and concordant changes in mRNA and protein abundance.

ABSTRACT:

Background

Psoriasis is a chronic disease characterized by the development of scaly red skin lesions and possible co-morbid conditions. The psoriasis lesional skin transcriptome has been extensively investigated, but mRNA levels do not necessarily reflect protein abundance. The purpose of this study was therefore to compare differential expression patterns of mRNA and protein in psoriasis lesions.

Methods

Lesional (PP) and uninvolved (PN) skin samples from 14 patients were analyzed using high-throughput complementary DNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results

We identified 4122 differentially expressed genes (DEGs) along with 748 differentially expressed proteins (DEPs). Global shifts in mRNA were modestly correlate

SUBMITTER: Swindell WR 

PROVIDER: S-EPMC4527112 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature