Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing.

ABSTRACT:

Background

Single-cell resequencing (SCRS) provides many biomedical advances in variations detection at the single-cell level, but it currently relies on whole genome amplification (WGA). Three methods are commonly used for WGA: multiple displacement amplification (MDA), degenerate-oligonucleotide-primed PCR (DOP-PCR) and multiple annealing and looping-based amplification cycles (MALBAC). However, a comprehensive comparison of variations detection performance between these WGA methods has not yet been performed.

Results

We systematically compared the advantages and disadvantages of different WGA methods, focusing particularly on variations detection. Low-coverage whole-genome sequencing revealed that DOP-PCR had the highest duplication ratio, but an even read distribution an

SUBMITTER: Hou Y 

PROVIDER: S-EPMC4527218 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature