Retinoic acid-induced CHD5 upregulation and neuronal differentiation of neuroblastoma.

ABSTRACT:

Background

Chromodomain-helicase DNA binding protein 5 (CHD5) is an important tumor suppressor gene deleted from 1p36.31 in neuroblastomas (NBs). High CHD5 expression is associated with a favorable prognosis, but deletion or low expression is frequent in high-risk tumors. We explored the role of CHD5 expression in the neuronal differentiation of NB cell lines.

Methods

NB cell lines SH-SY5Y (SY5Y), NGP, SK-N-DZ, IMR5, LAN5, SK-N-FI, NB69 and SH-EP were treated with 1-10 μM 13-cis-retinoic acid (13cRA) for 3-12 days. qRT-PCR and Western blot analyses were performed to measure mRNA and protein expression levels, respectively. Morphological differences were examined by both phase contrast and immunofluorescence studies.

Results

Treatment of SY5Y cells with 13cRA caused up

SUBMITTER: Higashi M 

PROVIDER: S-EPMC4527355 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature