Effect of the Materials Properties of Hydroxyapatite Nanoparticles on Fibronectin Deposition and Conformation.

ABSTRACT: Hydroxyapatite (HAP, Ca₁₀(PO₄)₆(OH)₂) nanoparticles with controlled materials properties have been synthesized through a two-step hydrothermal aging method to investigate fibronectin (Fn) adsorption. Two distinct populations of HAP nanoparticles have been generated: HAP1 particles had smaller size, plate-like shape, lower crystallinity, and more negative ζ potential than HAP2 particles. We then developed two-dimensional platforms containing HAP and Fn and analyzed both the amount and the conformation of Fn via Förster resonance energy transfer (FRET) at various HAP concentrations. Our FRET analysis reveals that larger amounts of more compact Fn molecules were adsorbed onto HAP1 than onto HAP2 particles. Additionally, our data show that the amount

SUBMITTER: Wu F 

PROVIDER: S-EPMC4527546 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature