An IPTG Inducible Conditional Expression System for Mycobacteria.

ABSTRACT: Conditional expression strains serve as a valuable tool to study the essentiality and to establish the vulnerability of a target under investigation in a drug discovery program. While essentiality implies an absolute requirement of a target function, vulnerability provides valuable information on the extent to which a target function needs to be depleted to achieve bacterial growth inhibition followed by cell death. The critical feature of an ideal conditional expression system is its ability to tightly regulate gene expression to achieve the full spectrum spanning from a high level of expression in order to support growth and near zero level of expression to mimic conditions of gene knockout. A number of bacterial conditional expression systems have been reported for use in mycobacteria.

SUBMITTER: Ravishankar S 

PROVIDER: S-EPMC4527713 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature