Prevention of nocturnal elevation of intraocular pressure by gene transfer of dominant-negative RhoA in rats.

ABSTRACT:

Importance

We developed a gene transfer tool for the control of nocturnal elevated intraocular pressure (IOP).

Objective

To demonstrate that inhibiting the trabecular meshwork RhoA pathway by delivering a mutated, dominant-negative RhoA gene (dnRhoA) carried inside a long-expressing recombinant virus would reduce nocturnal elevated IOP in a living animal.

Design and setting

We generated an optimized recombinant viral molecule by inserting a mutated RhoA complementary DNA with a translation enhancer-promoter into a specially designed plasmid containing mutated viral terminal repeats. We then generated the virus particle, self-complementary adeno-associated virus serotype 2 carrying the mutated gene (scAAV2.dnRhoA) and assessed its function in vitro by infecting primary

SUBMITTER: Borras T 

PROVIDER: S-EPMC4527865 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature