Size-specific follicle selection improves mouse oocyte reproductive outcomes.

ABSTRACT: Encapsulated in vitro follicle growth (eIVFG) has great potential to provide an additional fertility preservation option for young women and girls with cancer or other reproductive health threatening diseases. Currently, follicles are cultured for a defined period of time and analyzed as a cohort. However, follicle growth is not synchronous, and culturing follicles for insufficient or excessive times can result in compromised gamete quality. Our objective is to determine whether the selection of follicles based on size, rather than absolute culture time, better predict follicle maturity and oocyte quality. Multilayer secondary mouse follicles were isolated and encapsulated in 0.25% alginate. Follicles were cultured individually either for defined time periods or up to specific follicle dia

SUBMITTER: Xiao S 

PROVIDER: S-EPMC4527888 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature