A Vaccine Approach for the Prevention of Infections by Multidrug-resistant Enterococcus faecium.

ABSTRACT: The incidence of multidrug-resistant Enterococcus faecium hospital infections has been steadily increasing. With the goal of discovering new vaccine antigens, we systematically fractionated and purified four distinct surface carbohydrates from E. faecium endocarditis isolate Tx16, shown previously to be resistant to phagocytosis in the presence of human serum. The two most abundant polysaccharides consist of novel branched heteroglycan repeating units that include signature sugars altruronic acid and legionaminic acid, respectively. A minor high molecular weight polysaccharide component was recognized as the fructose homopolymer levan, and a glucosylated lipoteichoic acid (LTA) was identified in a micellar fraction. The polysaccharides were conjugated to the CRM197 carrier protein, and the

SUBMITTER: Kodali S 

PROVIDER: S-EPMC4528116 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature