Profiling the Proteome of Mycobacterium tuberculosis during Dormancy and Reactivation.

ABSTRACT: Tuberculosis, caused by Mycobacterium tuberculosis, still remains a major global health problem. The main obstacle in eradicating this disease is the ability of this pathogen to remain dormant in macrophages, and then reactivate later under immuno-compromised conditions. The physiology of hypoxic nonreplicating M. tuberculosis is well-studied using many in vitro dormancy models. However, the physiological changes that take place during the shift from dormancy to aerobic growth (reactivation) have rarely been subjected to a detailed investigation. In this study, we developed an in vitro reactivation system by re-aerating the virulent laboratory strain of M. tuberculosis that was made dormant employing Wayne's dormancy model, and compared the proteome profiles of dormant and reactivated bact

SUBMITTER: Gopinath V 

PROVIDER: S-EPMC4528245 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature