Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.

ABSTRACT: We report the current status of the FlyBase annotated gene set for Drosophila melanogaster and highlight improvements based on high-throughput data. The FlyBase annotated gene set consists entirely of manually annotated gene models, with the exception of some classes of small non-coding RNAs. All gene models have been reviewed using evidence from high-throughput datasets, primarily from the modENCODE project. These datasets include RNA-Seq coverage data, RNA-Seq junction data, transcription start site profiles, and translation stop-codon read-through predictions. New annotation guidelines were developed to take into account the use of the high-throughput data. We describe how this flood of new data was incorporated into thousands of new and revised annotations. FlyBase has adopted a philos

SUBMITTER: Matthews BB 

PROVIDER: S-EPMC4528329 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature