A Genetic Screen for Saccharomyces cerevisiae Mutants That Fail to Enter Quiescence.

ABSTRACT: Budding yeast begin the transition to quiescence by prolonging G1 and accumulating limited nutrients. They undergo asymmetric cell divisions, slow cellular expansion, acquire significant stress tolerance and construct elaborate cell walls. These morphologic changes give rise to quiescent (Q) cells, which can be distinguished from three other cell types in a stationary phase culture by flow cytometry. We have used flow cytometry to screen for genes that are required to obtain the quiescent cell fraction. We find that cell wall integrity is critical and these genes may help define quiescence-specific features of the cell wall. Genes required to evade the host innate immune response are common. These may be new targets for antifungal drugs. Acquired thermotolerance is also a common property,

SUBMITTER: Li L 

PROVIDER: S-EPMC4528334 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature