Simplified mechanistic models of gene regulation for analysis and design.

ABSTRACT: Simplified mechanistic models of gene regulation are fundamental to systems biology and essential for synthetic biology. However, conventional simplified models typically have outputs that are not directly measurable and are based on assumptions that do not often hold under experimental conditions. To resolve these issues, we propose a 'model reduction' methodology and simplified kinetic models of total mRNA and total protein concentration, which link measurements, models and biochemical mechanisms. The proposed approach is based on assumptions that hold generally and include typical cases in systems and synthetic biology where conventional models do not hold. We use novel assumptions regarding the 'speed of reactions', which are required for the methodology to be consistent with experimen

SUBMITTER: Hancock EJ 

PROVIDER: S-EPMC4528597 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature