The impact of chromatin remodelling on cellulase expression in Trichoderma reesei.

ABSTRACT:

Background

Trichoderma reesei is used for industry-scale production of plant cell wall-degrading enzymes, in particular cellulases, but also xylanases. The expression of the encoding genes was so far primarily investigated on the level of transcriptional regulation by regulatory proteins. Otherwise, the impact of chromatin remodelling on gene expression received hardly any attention. In this study we aimed to learn if the chromatin status changes in context to the applied conditions (repressing/inducing), and if the presence or absence of the essential transactivator, the Xylanase regulator 1 (Xyr1), influences the chromatin packaging.

Results

Comparing the results of chromatin accessibility real-time PCR analyses and gene expression studies of the two prominent cellulase-en

SUBMITTER: Mello-de-Sousa TM 

PROVIDER: S-EPMC4528718 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature