Fully automatic characterization and data collection from crystals of biological macromolecules.

ABSTRACT: Considerable effort is dedicated to evaluating macromolecular crystals at synchrotron sources, even for well established and robust systems. Much of this work is repetitive, and the time spent could be better invested in the interpretation of the results. In order to decrease the need for manual intervention in the most repetitive steps of structural biology projects, initial screening and data collection, a fully automatic system has been developed to mount, locate, centre to the optimal diffraction volume, characterize and, if possible, collect data from multiple cryocooled crystals. Using the capabilities of pixel-array detectors, the system is as fast as a human operator, taking an average of 6 min per sample depending on the sample size and the level of characterization required. Usin

SUBMITTER: Svensson O 

PROVIDER: S-EPMC4528805 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature