Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries).

ABSTRACT: Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0?Å resolution are reported.

SUBMITTER: Sakthivel D 

PROVIDER: S-EPMC4528930 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature