Structure of α-carbonic anhydrase from the human pathogen Helicobacter pylori.

ABSTRACT: The crystal structure of α-carbonic anhydrase, an enzyme present in the periplasm of Helicobacter pylori, a bacterium that affects humans and that is responsible for several gastric pathologies, is described. Two enzyme monomers are present in the asymmetric unit of the monoclinic space group P21, forming a dimer in the crystal. Despite the similarity of the enzyme structure to those of orthologues from other species, the H. pylori protein has adopted peculiar features in order to allow the bacterium to survive in the difficult environment of the human stomach. In particular, the crystal structure shows how the bacterium has corrected for the mutation of an essential amino acid important for catalysis using a negative ion from the medium and how it localizes close to the inner membrane in

SUBMITTER: Compostella ME 

PROVIDER: S-EPMC4528932 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature