Constraint of gene expression by the chromatin remodelling protein CHD4 facilitates lineage specification.

ABSTRACT: Chromatin remodelling proteins are essential for different aspects of metazoan biology, yet functional details of why these proteins are important are lacking. Although it is possible to describe the biochemistry of how they remodel chromatin, their chromatin-binding profiles in cell lines, and gene expression changes upon loss of a given protein, in very few cases can this easily translate into an understanding of how the function of that protein actually influences a developmental process. Here, we investigate how the chromatin remodelling protein CHD4 facilitates the first lineage decision in mammalian embryogenesis. Embryos lacking CHD4 can form a morphologically normal early blastocyst, but are unable to successfully complete the first lineage decision and form functional trophectoder

SUBMITTER: O'Shaughnessy-Kirwan A 

PROVIDER: S-EPMC4529036 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature