Apolipoprotein A-I mimetic peptide reverses impaired arterial healing after injury by reducing oxidative stress.

ABSTRACT:

Objective

Endothelial cell (EC) migration is essential for healing of arterial injuries caused by angioplasty, but a high cholesterol diet inhibits endothelial repair. In vivo studies suggest that apolipoprotein A-I (apoA-I), the major protein constituent of HDL, is essential for normal healing of arterial injuries. ApoA-I mimetics, including 4F, have been designed to mimic the amphipathic portion of the apoA-I molecule. This study was undertaken to determine if 4F improves endothelial migration and healing.

Methods

A razor scrape assay was used to analyze the effect of 4F on EC migration in vitro. Endothelial healing in vivo was assessed following electrical injury of carotid arteries in mice. Markers of oxidative stress were also examined.

Results

Lipid oxidation pr

SUBMITTER: Rosenbaum MA 

PROVIDER: S-EPMC4529116 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature