TGF-β induces fascin expression in gastric cancer via phosphorylation of smad3 linker area.

ABSTRACT:

Background

Fascin is an actin-bundling protein critical for tumor invasion. TGF-β could induce fascin expression in gastric cancer cells. In this study, we attempted to explore the role of p-smad3L in the expression of fascin induced by TGF-β in gastric cancer cells.

Methods

Pseudopodia were evaluated by immunofluorescence. Fascin expression was detected by RT-PCR and western blot. Smad3 siRNA was used to repress the endogenous smad3. The phosphorylations of smad3 linker region at sites s204, s208 and s213 were detected by western blot. The fascin promoter reporter activity was measured by dual luciferase assay.

Results

TGF-β could increase the formation of pseudopodia and the expression of fascin in gastric cancer cells. Smad3 depletion abrogated the expression of fa

SUBMITTER: Li L 

PROVIDER: S-EPMC4529611 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature