Murine Double-Minute 2 Homolog Single Nucleotide Polymorphisms 285 and 309 in Cervical Carcinogenesis.

ABSTRACT:

Background and objective

In Caucasians, the MDM2 single nucleotide polymorphism (SNP) 285 G>C (rs117039649) neutralizes the effect of 309 T>G (rs2279744), which increases MDM2 expression and impairs the p53 pathway. In this study, we examined the distribution of these two SNPs in Polish women with squamous cell carcinoma (SCC) (n = 379), adenocarcinoma (n = 59) and other cervical tumor types (n = 18).

Methods

The polymerase chain reaction-restriction fragment length polymorphism technique and DNA sequencing were employed in our study.

Results

The P trend value calculated for the MDM2 285 G>C polymorphism was statistically significant (P trend = 0.016) for SCC. Using logistical regression analysis adjusted for the effect of age, pregnancy, oral contraceptive use, tobac

SUBMITTER: Roszak A 

PROVIDER: S-EPMC4529876 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature