Epigenetic profiles of pre-diabetes transitioning to type 2 diabetes and nephropathy.

ABSTRACT:

Aim

To examine DNA methylation profiles in a longitudinal comparison of pre-diabetes mellitus (Pre-DM) subjects who transitioned to type 2 diabetes mellitus (T2DM).

Methods

We performed DNA methylation study in bisulphite converted DNA from Pre-DM (n = 11) at baseline and at their transition to T2DM using Illumina Infinium HumanMethylation27 BeadChip, that enables the query of 27578 individual cytosines at CpG loci throughout the genome, which are focused on the promoter regions of 14495 genes.

Results

There were 694 CpG sites hypomethylated and 174 CpG sites hypermethylated in progression from Pre-DM to T2DM, representing putative genes involved in glucose and fructose metabolism, inflammation, oxidative and mitochondrial stress, and fatty acid metabolism. These resu

SUBMITTER: VanderJagt TA 

PROVIDER: S-EPMC4530325 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature