The human urothelial tight junction: claudin 3 and the ZO-1α⁺ switch.

ABSTRACT:

Objective

Tight junctions are multicomponent structures, with claudin proteins defining paracellular permeability. Claudin 3 is a candidate for the exceptional "tightness" of human urothelium, being localised to the terminal tight junction (TJ) of superficial cells. Our aim was to determine whether claudin 3 plays an instigating and/or a functional role in the urothelial TJ.

Materials and methods

Normal human urothelial (NHU) cells maintained as non-immortalised cell lines were retrovirally-transduced to over-express or silence claudin 3 expression. Stable sublines induced to stratify or differentiate were assessed for TJ formation by immunocytochemistry and transepithelial electrical resistance (TER). Expression of claudin 3, ZO-1 and ZO-1α⁺ was examined in nativ

SUBMITTER: Smith NJ 

PROVIDER: S-EPMC4530542 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature