Protein kinase A activity is necessary for fission and fusion of Golgi to endoplasmic reticulum retrograde tubules.

ABSTRACT: It is becoming increasingly accepted that together with vesicles, tubules play a major role in the transfer of cargo between different cellular compartments. In contrast to our understanding of the molecular mechanisms of vesicular transport, little is known about tubular transport. How signal transduction molecules regulate these two modes of membrane transport processes is also poorly understood. In this study we investigated whether protein kinase A (PKA) activity regulates the retrograde, tubular transport of Golgi matrix proteins from the Golgi to the endoplasmic reticulum (ER). We found that Golgi-to-ER retrograde transport of the Golgi matrix proteins giantin, GM130, GRASP55, GRASP65, and p115 was impaired in the presence of PKA inhibitors. In addition, we unexpectedly found accumul

SUBMITTER: Tenorio MJ 

PROVIDER: S-EPMC4530959 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature