Function and diversity of P0 proteins among cotton leafroll dwarf virus isolates.

ABSTRACT:

Background

The RNA silencing pathway is an important anti-viral defense mechanism in plants. As a counter defense, some members of the viral family Luteoviridae are able to evade host immunity by encoding the P0 RNA silencing suppressor protein. Here we explored the functional diversity of P0 proteins among eight cotton leafroll dwarf virus (CLRDV) isolates, a virus associated with a worldwide cotton disease known as cotton blue disease (CBD).

Methods

CLRDV-infected cotton plants of different varieties were collected from five growing fields in Brazil and their P0 sequences compared to three previously obtained isolates. P0's silencing suppression activities were scored based on transient expression experiments in Nicotiana benthamiana leaves.

Results

High sequence di

SUBMITTER: Cascardo RS 

PROVIDER: S-EPMC4531488 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature