Increased DNA damage and repair deficiency in granulosa cells are associated with ovarian aging in rhesus monkey.

ABSTRACT:

Purpose

Ovarian aging is closely tied to the decline in ovarian follicular reserve and oocyte quality. During the prolonged reproductive lifespan of the female, granulosa cells connected with oocytes play critical roles in maintaining follicle reservoir, oocyte growth and follicular development. We tested whether double-strand breaks (DSBs) and repair in granulosa cells within the follicular reservoir are associated with ovarian aging.

Methods

Ovaries were sectioned and processed for epi-fluorescence microscopy, confocal microscopy, and immunohistochemistry. DNA damage was revealed by immunstaining of γH2AX foci and telomere damage by γH2AX foci co-localized with telomere associated protein TRF2. DNA repair was indicated by BRCA1 immunofluorescence.

Results

DSBs in gr

SUBMITTER: Zhang D 

PROVIDER: S-EPMC4531862 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature