Role of ADAM17 in the non-cell autonomous effects of oncogene-induced senescence.

ABSTRACT:

Introduction

Cellular senescence is a terminal cell proliferation arrest that can be triggered by oncogenes. One of the traits of oncogene-induced senescence (OIS) is the so-called senescence-associated secretory phenotype or senescence secretome. Depending on the context, the non-cell autonomous effects of OIS may vary from tumor suppression to promotion of metastasis. Despite being such a physiological and pathologically relevant effector, the mechanisms of generation of the senescence secretome are largely unknown.

Methods

We analyzed by label-free proteomics the secretome of p95HER2-induced senescent cells and compared the levels of the membrane-anchored proteins with their transcript levels. Then, protein and RNA levels of ADAM17 were evaluated by using Western blot and

SUBMITTER: Morancho B 

PROVIDER: S-EPMC4532141 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature