PH measurement as quality control on human post mortem brain tissue: a study of the BrainNet Europe consortium.

ABSTRACT:

Aims

Most brain diseases are complex entities. Although animal models or cell culture experiments mimic some disease aspects, human post mortem brain tissue remains essential to advance our understanding of brain diseases using biochemical and molecular techniques. Post mortem artefacts must be properly understood, standardized, and either eliminated or factored into such experiments. Here we examine the influence of several premortem and post mortem factors on pH, and discuss the role of pH as a biochemical marker for brain tissue quality.

Methods

We assessed brain tissue pH in 339 samples from 116 brains provided by 8 different European and 2 Australian brain bank centres. We correlated brain pH with tissue source, post mortem delay, age, gender, freezing method, storage d

SUBMITTER: Monoranu CM 

PROVIDER: S-EPMC4532399 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature