Obesity alters the peripheral circadian clock in the aorta and microcirculation.

ABSTRACT:

Objective

Perturbation of daily rhythm increases cardiovascular risk. The aim of this study was to determine whether obesity alters circadian gene expression and microvascular function in lean mice and obese (db/db) mice.

Methods

Mice were subjected to normal LD or DD to alter circadian rhythm. Metabolic parameters and microvascular vasoreactivity were evaluated. Array studies were conducted in the am and pm cycles to assess the rhythmicity of the entire genomics. Rhythmic expression of specific clock genes (Bmal1, Clock, Npas2, Per1, Per2, and Cry1), clock output genes (dbp), and vascular relaxation-related genes (eNOS, GTPCH1) were assessed.

Results

Obesity was associated with metabolic dysfunction and impaired endothelial dilation in the microvasculature. Circadian

SUBMITTER: Nernpermpisooth N 

PROVIDER: S-EPMC4532430 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature