Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.

ABSTRACT: Synthetic regulatory circuits encoded in RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity. We show that the circuits function in mammalian cells when encoded in modified mRNA or self-replicating RNA.

SUBMITTER: Wroblewska L 

PROVIDER: S-EPMC4532950 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature