The use of next generation sequencing in the diagnosis and typing of respiratory infections.

ABSTRACT:

Background

Molecular assays are the gold standard methods used to diagnose viral respiratory pathogens. Pitfalls associated with this technique include limits to the number of targeted pathogens, the requirement for continuous monitoring to ensure sensitivity/specificity is maintained and the need to evolve to include emerging pathogens. Introducing target independent next generation sequencing (NGS) could resolve these issues and revolutionise respiratory viral diagnostics.

Objectives

To compare the sensitivity and specificity of target independent NGS against the current standard diagnostic test.

Study design

Diagnostic RT-PCR of clinical samples was carried out in parallel with target independent NGS. NGS sequences were analyzed to determine the proportion with vir

SUBMITTER: Thorburn F 

PROVIDER: S-EPMC4533236 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature