A high-resolution physical map integrating an anchored chromosome with the BAC physical maps of wheat chromosome 6B.

ABSTRACT:

Background

A complete genome sequence is an essential tool for the genetic improvement of wheat. Because the wheat genome is large, highly repetitive and complex due to its allohexaploid nature, the International Wheat Genome Sequencing Consortium (IWGSC) chose a strategy that involves constructing bacterial artificial chromosome (BAC)-based physical maps of individual chromosomes and performing BAC-by-BAC sequencing. Here, we report the construction of a physical map of chromosome 6B with the goal of revealing the structural features of the third largest chromosome in wheat.

Results

We assembled 689 informative BAC contigs (hereafter reffered to as contigs) representing 91% of the entire physical length of wheat chromosome 6B. The contigs were integrated into a radiation hy

SUBMITTER: Kobayashi F 

PROVIDER: S-EPMC4534020 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature