Meristem micropropagation of cassava (Manihot esculenta) evokes genome-wide changes in DNA methylation.

ABSTRACT: There is great interest in the phenotypic, genetic and epigenetic changes associated with plant in vitro culture known as somaclonal variation. In vitro propagation systems that are based on the use of microcuttings or meristem cultures are considered analogous to clonal cuttings and so widely viewed to be largely free from such somaclonal effects. In this study, we surveyed for epigenetic changes during propagation by meristem culture and by field cuttings in five cassava (Manihot esculenta) cultivars. Principal Co-ordinate Analysis of profiles generated by methylation-sensitive amplified polymorphism revealed clear divergence between samples taken from field-grown cuttings and those recovered from meristem culture. There was also good separation between the tissues of field samples but t

SUBMITTER: Kitimu SR 

PROVIDER: S-EPMC4534864 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature