Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.

ABSTRACT:

Background

In order to provide a system fully integrated with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMOs in food/feed matrices, two bidirectional DNA walking methods targeting p35S or tNOS, the most common transgenic elements found in GM crops, were developed. These newly developed DNA walking methods are completing the previously implemented DNA walking method targeting the t35S pCAMBIA element.

Methods

Food/feed matrices containing transgenic crops (Bt rice or MON863 maize) were analysed using the integrated DNA walking system.

Results

First, the newly developed DNA walking methods, anchored on the sequences used for the p35S or tNOS qPCR screening, were tested on Bt rice

SUBMITTER: Fraiture MA 

PROVIDER: S-EPMC4535744 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature