Bacterial Landscape of Bloodstream Infections in Neutropenic Patients via High Throughput Sequencing.

ABSTRACT:

Background

Bloodstream infection (BSI) is a common and potentially life-threatening complication in patients with hematological malignancies and therapy-induced neutropenia. Administration of broad spectrum antibiotics has substantially decreased the mortality rate in febrile neutropenia, but bacterial infection is documented in only one-third or fewer of the cases. BSI is typically diagnosed by blood culture; however, this method can detect only culturable pathogens.

Methods

In the present study, a total of 130 blood samples from hematological patients receiving dose-intensive antitumoural treatment were subjected to 16S rRNA PCR and 62 of them were cultured. PCR positive samples were processed to high throughput sequencing by amplifying the V1-V3 regions of the 16S rRNA ge

SUBMITTER: Gyarmati P 

PROVIDER: S-EPMC4536222 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature