Identification of novel genes that regulate androgen receptor signaling and growth of androgen-deprived prostate cancer cells.

ABSTRACT: Prostate cancer progression to castration refractory disease is associated with anomalous transcriptional activity of the androgen receptor (AR) in an androgen-depleted milieu. To identify novel gene products whose downregulation transactivates AR in prostate cancer cells, we performed a screen of enzymatically-generated shRNA lenti-libraries selecting for transduced LNCaP cells with elevated expression of a fluorescent reporter gene under the control of an AR-responsive promoter. The shRNAs present in selected populations were analyzed using high-throughput sequencing to identify target genes. Highly enriched gene targets were then validated with siRNAs against selected genes, testing first for increased expression of luciferase from an AR-responsive promoter and then for altered expressi

SUBMITTER: Levina E 

PROVIDER: S-EPMC4537001 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature