Unbiased Quantitative Models of Protein Translation Derived from Ribosome Profiling Data.

ABSTRACT: Translation of RNA to protein is a core process for any living organism. While for some steps of this process the effect on protein production is understood, a holistic understanding of translation still remains elusive. In silico modelling is a promising approach for elucidating the process of protein synthesis. Although a number of computational models of the process have been proposed, their application is limited by the assumptions they make. Ribosome profiling (RP), a relatively new sequencing-based technique capable of recording snapshots of the locations of actively translating ribosomes, is a promising source of information for deriving unbiased data-driven translation models. However, quantitative analysis of RP data is challenging due to high measurement variance and the inabilit

SUBMITTER: Gritsenko AA 

PROVIDER: S-EPMC4537299 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature